Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer's Patch dendritic cells that express lysozyme.
Résumé
BACKGROUND & AIMS:: Lysozyme has an important role in preventing bacterial infection. In the gastrointestinal tract, lysozyme is thought to be mainly expressed by Paneth cells of the crypt epithelium. We investigated its expression in the Peyer's patch-a major intestinal site of antigen sampling and pathogen entry. METHODS:: We performed immunostaining on normal and Salmonella Typhimurium-infected intestinal samples and analyzed them by confocal microscopy and flow cytometry. RESULTS:: In Peyer's patch of mouse, rat, and human, lysozyme was strongly expressed in the germinal center of follicles by tingible body macrophages and in the subepithelial dome by a subset of myeloid dendritic cells (DC). Among DC subsets from mouse Peyer's patches, these lysozyme-expressing DC displayed the highest surface expression of class II major histocompatibility complex and co-stimulatory molecules; they were the most efficient at capturing microspheres in vitro. Moreover, they were the main DC subset involved in bacterial pathogen uptake and in dead cell clearance, including M cells. CONCLUSION:: The subepithelial dome of Peyer's patches contains a unique population of intestinal DC that secrete high levels of lysozyme and internalize bacteria and dead cells.