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Article Dans Une Revue Enzyme and Microbial Technology Année : 2009

Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug

Résumé

Here we present a simple bioreactor with immobilized rat hepatic microsomal membranes which may be applicable in screening and prediction of reactive intermediates (RI) using alumina membrane as a support. To monitor the effectiveness of our technique, acetaminophen (AP) already known to produce RI has been utilized as a model compound. The resulting immobilized enzyme membrane (IEM) was able to produce reasonable amount of glutathione conjugates within a residence time of seconds. At the other end biosensor was tailored to predict RI by relating the enhanced activities of microsomal glutathione S-transferase (mGST) as a marker of drug and chemical stress.

Domaines

Chimie
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Dates et versions

hal-00419740 , version 1 (24-09-2009)

Identifiants

Citer

Shazia Tanvir, Hervé Adenier, Sylviane Pulvin. Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug. Enzyme and Microbial Technology, 2009, 45 (2), pp.112-117. ⟨10.1016/j.enzmictec.2009.05.006⟩. ⟨hal-00419740⟩
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