Global analysis of extracytoplasmic stress signaling in Escherichia coli

Stéphanie Bury-Moné (1) , Yanoura Nomane (1) , Nancie Reymond (2) , Romain Barbet (3) , Eric Jacquet (3) , Sandrine Imbeaud (2) , Annick Jacq (1) , Philippe Bouloc (1)

1 IGM - Institut de génétique et microbiologie [Orsay]
2 CGM - Centre de génétique moléculaire
3 ICSN - Institut de Chimie des Substances Naturelles

The Bae, Cpx, Psp, Rcs, and σE pathways constitute the Escherichia coli signaling systems that detect and respond to alterations of the bacterial envelope. Contributions of these systems to stress response have previously been examined individually; however, the possible interconnections between these pathways are unknown. Here we investigate the dynamics between the five stress response pathways by determining the specificities of each system with respect to signalinducing conditions, and monitoring global transcriptional changes in response to transient overexpression of each of the effectors. Our studies show that different extracytoplasmic stress conditions elicit a combined response of these pathways. Involvement of the five pathways in the various tested stress conditions is explained by our unexpected finding that transcriptional responses induced by the individual systems show little overlap. The extracytoplasmic stress signaling pathways in E. coli thus regulate mainly complementary functions whose discrete contributions are integrated to mount the full adaptive response. © 2009 Bury-Moné et al.

Mots clés: small noncoding rnas; gene-expression; shock-protein response; 2-component regulatory system; outer-membrane proteins; genome-wide analyses; dnaj-like protein; rcs phosphorelay; sigma(e) regulon; cell-envelope

Domaines: Bactériologie

Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, et al.. Global analysis of extracytoplasmic stress signaling in Escherichia coli. PLoS Genetics, 2009, 5 (9), pp.e1000651. ⟨10.1371/journal.pgen.1000651⟩. ⟨hal-00418796⟩