Dimeric Transferrin Inhibits Phagocytosis of Residual Bodies by Testicular Rat Sertoli Cells1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of Reproduction Année : 2008

Dimeric Transferrin Inhibits Phagocytosis of Residual Bodies by Testicular Rat Sertoli Cells1

Résumé

Transferrin is well known as an iron transport glycoprotein. Dimeric or tetrameric transferrin forms have recently been reported to modulate phagocytosis by human leukocytes. It is mainly synthesized by the liver, and also by other sources, such as Sertoli cells of the testis. Sertoli cells show a strong phagocytic activity toward apoptotic germ cells and residual bodies. Here, we provide evidence that purified human dimeric transferrin from commercial sources decreased residual body phagocytosis, unlike monomeric transferrin. The presence of iron appeared essential for dimeric transferrin inhibitory activity. Importantly, dimeric transferrin could be visualized by immunoblotting in Sertoli cell lysates as well as in culture media, indicating that dimeric transferrin could be physiologically secreted by Sertoli cells. By siRNA-mediated knockdown, we show that endogenous transferrin significantly inhibited residual body ingestion by Sertoli cells. These results are the first to identify dimeric transferrin in Sertoli cells and to demonstrate its implication as a physiological modulator of residual body phagocytosis by Sertoli cells.
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Dates et versions

hal-00413598 , version 1 (04-09-2009)

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Marina G. Yefimova, Amina Sow, Isabelle Fontaine, Vincent Guilleminot, Nadine Martinat, et al.. Dimeric Transferrin Inhibits Phagocytosis of Residual Bodies by Testicular Rat Sertoli Cells1. Biology of Reproduction, 2008, 78 (4), pp.697-704. ⟨10.1095/biolreprod.107.063107⟩. ⟨hal-00413598⟩
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