Adsorption and desorption of monomeric Bt (Bacillus thuringiensis) Cry1Aa toxin on montmorillonite and kaolinite

N. Helassa (1) , H. Quiquampoix (1) , S. Noinville (2) , W. Szponarski (3) , Siobhan Staunton (1)

1 UMR Eco&Sols - Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes
2 LADIR - Laboratoire de Dynamique Interactions et Réactivité
3 BPMP - Biochimie et Physiologie Moléculaire des Plantes

Genetically modified crops, which produce pesticidal proteins from Bacillus thuringiensis, release the toxins into soils through root exudates and upon decomposition of crop residues. Although the phenomena of gene transfer and emergence of resistance have been well documented, the fate of these toxins in sail has not yet been clearly elucidated. The aim of this study was to elucidate the adsorption and the desorbability of the Cry1Aa Bt insecticidal protein in contact with two sodium-saturated clays: montmorillonite and kaolinite. Because the toxin is released into soil in small quantities, it was assumed that it will be in a monomeric state in solution until it oligomerized on cell membranes. The originality of this study was to focus on the monomeric form of the protein. Specific sample conditions were required to avoid polymerisation. A pH above 6.5 and an ionic strength of at least 150 mM (NaCl) were necessary to keep the protein in solution and in a monomeric state. The adsorption isotherms obtained were of the L-type (low affinity) for both clays and fitted the Langmuir equation. The adsorption maximum of the toxin, calculated by the Langmuir nonlinear regression, decreased with increasing pH from 6.5, which was close to the isoelectric point, to 9. At pH 6.5, the calculated adsorption was 1.7 g g-l on montmorillonite and 0.04 g g-l on kaolinite. Desorbability measurements showed that a small fraction of toxin could be desorbed by water (up to 14%) and more by alkaline pH buffers (36 +/- 7%), indicating that it was not tightly bound. Numerous surfactants were evaluated and the toxin was found to be easily desorbed from both clays when using zwitterionic and nonionic surfactants; such as CHAPS, Triton-X-100, and Tween 20. This finding has important implications for the optimization of detection methods for Bt toxin in soil. (C) 2009 Elsevier Ltd. All rights reserved.

Mots clés: Bacillus thuringiensis; Adsorption; Insecticidal protein; Cry1Aa; Desorption; Montmorillonite; Kaolinite; Surfactant; Detergent extracellular enzyme-activity; bovine serum-albumin; ovine prion protein; subsp kurstaki; insecticidal activity; mineral surfaces; transgenic plants; delta-endotoxin; clay-minerals; humic acids

Domaines: Biologie végétale

N. Helassa, H. Quiquampoix, S. Noinville, W. Szponarski, Siobhan Staunton. Adsorption and desorption of monomeric Bt (Bacillus thuringiensis) Cry1Aa toxin on montmorillonite and kaolinite. Soil Biology and Biochemistry, 2009, 41 (3), pp.498-504. ⟨10.1016/j.soilbio.2008.12.008⟩. ⟨hal-00408995⟩