Localizing proteins by tissue printing.

Rafael F Pont-Lezica (1)

1 SCSV - Surfaces Cellulaires et Signalisation chez les Végétaux

The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins, nucleic acids, carbohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins, probably the most frequently used application, but the localization of other molecules is reported and the sources indicated.

Mots clés: Western blotting.; Immunodetection; tissue blotting; protein localization; Western blotting

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Rafael F Pont-Lezica. Localizing proteins by tissue printing.. Methods in Molecular Biology, 2009, 536, pp.75-88. ⟨10.1007/978-1-59745-542-8_10⟩. ⟨hal-00402326⟩