Escherichia Coli functionalized magnetic nanobeads as an ultrasensitive biosensor for heavy metals

Mina Souiri (1) , Ibtissem Gammoudi (1) , Hafedh Ben Ouada (2) , Laurent Mora (3) , Thierry Jouenne (4) , Nicole Jaffrezic-Renault (5) , Corinne Dejous (6) , Ali Othmane (1) , Anthony Duncan (4)

1 Laboratoire de Biophysique [Monastir]
2 LPCI - Laboratoire de Physique et Chimie des Interfaces [Monastir]
3 TREFLE - Transferts, écoulements, fluides, énergétique
4 PBM - Polymères, biopolymères, membranes
5 SA - Sciences Analytiques
6 IMS - Laboratoire de l'intégration, du matériau au système

The detection of traces of heavy metals in real environmental samples is a difficult task. In this work, we combined the advantages of nanobeads' (NBs) properties with the simplicity of the layer-by-layer procedure, along with the use of micro-organisms as bioreceptors, for the elaboration of a novel electrochemical biosensor based on nanocomposite films. The whole cells (Escherichia. coli) K-12 used as bioreceptors were fixed onto a surface of indium-tin-oxide (ITO) glass with and without NBs and/or polyelectrolyte multilayers (PEM). Using the electrochemical impedance spectroscopy (EIS) technique, cadmium and mercury were detected.

Mots clés: Biosensor; nanobeads; PEM; heavy metals; Escherichia Coli; EIS

Domaines: Micro et nanotechnologies/Microélectronique; Matériaux; Biotechnologies; Biotechnologie

Mina Souiri, Ibtissem Gammoudi, Hafedh Ben Ouada, Laurent Mora, Thierry Jouenne, et al.. Escherichia Coli functionalized magnetic nanobeads as an ultrasensitive biosensor for heavy metals. Eurosensors XXIII, Sep 2009, Lausanne, Switzerland. pp.1027-1030, ⟨10.1016/j.proche.2009.07.256⟩. ⟨hal-00401501⟩