Skip to Main content Skip to Navigation
Journal articles

Biological activity and binding site characteristics of the PA1b entomotoxin on insects from different orders

Abstract : The aim of this work was to investigate both the biological activity of an entomotoxin, the pea albumin 1b (PA1b), and the presence or absence of its binding site within an array of insect species. The data obtained showed that insect sensitivity was not related to its taxonomic position. Moreover, PA1b was not toxic to several tested microorganisms. However, the binding site was found to be conserved among very different insects, displaying similar thermodynamic constants regardless of the in vivo species sensitivity. The binding site alone was, therefore, not sufficient for toxicity. One exception was the pea weevil, Bruchus pisorum, which was the only tested species without any detectable binding activity. These findings indicate that the binding site probably has an important endogenous function in insects and that adaptation to pea seeds resulted in the elimination of the toxin binding activity in two independent insect lineages. Other mechanisms are likely to interact with the toxin effects, although they are still largely unknown, but there is no evidence of any specific degradation of PA1b in the midgut of insects insensitive to the toxin, such as Drosophila melanogaster or Mamestra brassicae.
Keywords : toxin PA1b binding site
Complete list of metadatas

Cited literature [28 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00391325
Contributor : Insa Laboratoire Bf2i <>
Submitted on : Friday, April 26, 2019 - 12:28:27 PM
Last modification on : Sunday, May 31, 2020 - 2:02:21 AM

File

GressentF_DuportG_RahiouiI_EtA...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

F. Gressent, G. Duport, I. Rahioui, Y. Pauchet, P. Bolland, et al.. Biological activity and binding site characteristics of the PA1b entomotoxin on insects from different orders. Journal of Insect Science, Oxford University Press, 2007, 7 (1), pp.12. ⟨10.1673/031.007.1201⟩. ⟨hal-00391325⟩

Share

Metrics

Record views

180

Files downloads

195