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PscF is a major component of the Pseudomonas aeruginosa type III secretion needle.

Abstract : Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, translocates exoenzymes (Exo) directly into the eukaryotic cell cytoplasm. This is accomplished by a type III secretion/translocation machinery. Here, we show that the P. aeruginosa type III secretory needle structure is composed essentially of PscF, a protein required for secretion and P. aeruginosa cytotoxicity. Partially purified needles, detached from the bacterial surface, are 60-80 nm in length and 7 nm in width, resembling needles from Yersinia spp.. YscF of Yersinia enterocolitica was able to functionally complement the pscF deletion, but required 11 P. aeruginosa-specific amino acids at the N-terminus for its function.
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https://hal.archives-ouvertes.fr/hal-00378748
Contributor : Ina Attree <>
Submitted on : Sunday, April 26, 2009 - 6:13:45 PM
Last modification on : Monday, April 6, 2020 - 9:22:29 AM

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Alexandrine Pastor, Jacqueline Chabert, Mathilde Louwagie, Jerôme Garin, Ina Attree. PscF is a major component of the Pseudomonas aeruginosa type III secretion needle.. FEMS Microbiology Letters, Wiley-Blackwell, 2005, 253 (1), pp.95-101. ⟨10.1016/j.femsle.2005.09.028⟩. ⟨hal-00378748⟩

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