Counterions release from electrostatic complexes of polyelectrolytes and proteins of opposite charge : a direct measurement

Jérémie Gummel (1) , Fabrice Cousin (1) , François Boué (1)

1 LLB - UMR 12 - Laboratoire Léon Brillouin

Though often considered as one of the main driving process of the complexation of species of opposite charges, the release of counterions has never been experimentally directly measured on polyelectrolyte/proteins complexes. We present here the first structural determination of such a release by Small Angle Neutron Scattering in complexes made of lysozyme, a positively charged protein and of PSS, a negatively charged polyelectrolyte. Both components have the same neutron density length, so their scattering can be switched off simultaneously in an appropriate "matching" solvent; this enables determination of the spatial distribution of the single counterions within the complexes. The counterions (including the one subjected to Manning condensation) are expelled from the cores where the species are at electrostatic stoichiometry.

Mots clés: polyelectrolytes; small angle neutron scattering

Domaines: Matière Molle [cond-mat.soft]; Polymères

Jérémie Gummel, Fabrice Cousin, François Boué. Counterions release from electrostatic complexes of polyelectrolytes and proteins of opposite charge : a direct measurement. Journal of the American Chemical Society, 2007, 129 (18), pp.5806-5807. ⟨hal-00371545⟩