Skip to Main content Skip to Navigation
Journal articles

ERK5 Knockdown generates mouse leukemia cells with low MHC class i levels that activate NK cells and block tumorigenesis.

Abstract : Tumor cell-based vaccines are currently used in clinical trails, but they are in general poorly immunogenic because they are composed of cell extracts or apoptotic cells. Live tumor cells should be much better Ags provided that they are properly processed by the host immune system. We show herein that stable expression of a small hairpin RNA for ERK5 (shERK5) decreases ERK5 levels in human and mouse leukemic cells and leads to their elimination by NK cells in vivo. The shERK5 cells show down-regulation of MHC class I expression at the plasma membrane. Accordingly, ectopic activation of the ERK5 pathway induces MHC class I gene expression. Coinjection of shERK5-expressing cells into the peritoneum diminishes survival of engrafted wild-type tumor cells. Moreover, s.c. injection of shERK5-expressing cells strongly diminishes tumor development by wild-type cells. Our results show that shERK5 expression in leukemia cells effectively attenuates their tumor activity and allows their use as a tumor cell-based vaccine.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00368654
Contributor : Sylvie Julien <>
Submitted on : Tuesday, March 17, 2009 - 10:45:17 AM
Last modification on : Thursday, February 14, 2019 - 3:33:33 PM

Links full text

Identifiers

Collections

Citation

Seyma Charni, Juan Ignacio Aguilo, Johan Garaude, Geoffroy de Bettignies, Chantal Jacquet, et al.. ERK5 Knockdown generates mouse leukemia cells with low MHC class i levels that activate NK cells and block tumorigenesis.. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2009, 182 (6), pp.3398-405. ⟨10.4049/jimmunol.0803006⟩. ⟨hal-00368654⟩

Share

Metrics

Record views

184