Evaluation of functional gene enrichment in a soil metagenomic clone library

Sandrine Demanèche (1) , Maude M. David (1) , Elisabeth Navarro (1) , Pascal Simonet (1) , Timothy M Vogel (1)

1 AMPERE - Ampère

We evaluated the use of mixed oligonucleotide probes hybridized to metagenomic clones spotted on high density membranes. The pooled probes included oligonucleotides designed for genes associated with denitrification, antibiotic resistance, and dehalogenation among others. Pyrosequence comparison between the clones and the original DNA demonstrated the utility of clone screening with pooled probes.

Mots clés: PCR; DNA; DEGRADATION; Metagenomic; Pyrosequencing; Screening; DENITRIFYING BACTERIA; NOSZ GENES; NIRK

Domaines: Biodiversité et Ecologie

Sandrine Demanèche, Maude M. David, Elisabeth Navarro, Pascal Simonet, Timothy M Vogel. Evaluation of functional gene enrichment in a soil metagenomic clone library. Journal of Microbiological Methods, 2009, 76 (1), pp.105-107. ⟨10.1016/j.mimet.2008.09.009⟩. ⟨hal-00368291⟩