Decalactone production by Yarrowia lipolytica under increased O2 transfer rates.

M. Aguedo , N. Gomes , E Escamilla Garcia (1, 2, 3) , Yves Waché (1, 3) , M. Mota (4) , J. A. Teixeira (5) , I. Belo

1 GPMA - Génie des Procédés Microbiologiques et Alimentaires
2 Department of Endocrinology
3 Laboratoire de Microbiologie
4 IBB - Institute for Biotechnology and Bioengineering
5 Dept. Elect. & Comp. Eng.

Yarrowia lipolytica converts methyl ricinoleate to gamma-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-gamma-decalactone produced by the yeast (263 mg l(-1)) occurred by increasing the k(L)a up to 120 h(-1) at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3-hydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e., under increased O(2) solubility, but, although growth was accelerated, gamma-decalactone production decreased. However, by applying 0.5 MPa during growth and biotransformation gave increased concentrations of dec-2-en-4-olide and dec-3-en-4-olide (70 mg l(-1)).

Mots clés: 3-Hydroxyacyl CoA Dehydrogenases; Lactones; Oxygen; Ricinoleic Acids; Yarrowia

Mots clés: Biotransformation

Domaines: Biotechnologie; Biochimie, Biologie Moléculaire

M. Aguedo, N. Gomes, E Escamilla Garcia, Yves Waché, M. Mota, et al.. Decalactone production by Yarrowia lipolytica under increased O2 transfer rates.. Biotechnology Letters, 2005, 27 (20), pp.1617-21. ⟨10.1007/s10529-005-2517-z⟩. ⟨hal-00368199⟩