Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiO_x interfaces

Maël Manesse (1) , Valérie Stambouli (2) , Rabah Boukherroub (3, 4) , Sabine Szunerits (1, 3, 4)

1 LEPMI - Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces
2 LMGP - Laboratoire des matériaux et du génie physique
3 IRI - Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq]
4 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520

The use of Au/SiO_x interfaces for the investigation of DNA hybridization using electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) simultaneously is demonstrated. Standard glass chemistry was used to link single-stranded DNA (ss-DNA) on aldehyde-terminated Au/SiO_x interfaces. The layer thickness and amount of grafted oligonucleotides (ODNs) were calculated from SPR on the basis of a multilayer system of glass/Ti/Au/SiO_x/grafted molecule. Capacitance and resistance values of the modified interface before and after hybridization were calculated from EIS data using an equivalent circuit and allowed the affinity rate constant, K_A = 4.07 × 10⁵ M^-1, to be determined. The EIS results were comparable to those obtained by SPR hybridization kinetics recorded in parallel.

Domaines: Matériaux

Maël Manesse, Valérie Stambouli, Rabah Boukherroub, Sabine Szunerits. Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiO_x interfaces. Analyst, 2008, 133 (8), pp. 1097-1103. ⟨10.1039/b804825h⟩. ⟨hal-00365585⟩