The C-Terminal Domain of Cernunnos/XLF Is Dispensable for DNA Repair In Vivo

Laurent Malivert (1, 2) , Isabelle Callebaut (3) , Paola Rivera-Munoz (1, 2) , Alain Fischer (1, 4, 5) , Jean-Paul Mornon (3) , Patrick Revy (1, 4) , Jean-Pierre de Villartay (1, 4, 5)

1 Developpement Normal et Pathologique du Système Immunitaire
2 UPD7 - Université Paris Diderot - Paris 7
3 IMPMC - Institut de minéralogie et de physique des milieux condensés
4 UPD5 - Université Paris Descartes - Paris 5
5 Hôpital Necker - Enfants Malades [AP-HP]

The core nonhomologous end-joining DNA repair pathway is composed of seven factors: Ku70, Ku80, DNA-PKcs, Artemis, XRCC4 (X4), DNA ligase IV (L4), and Cernunnos/XLF (Cernunnos). Although Cernunnos and X4 are structurally related and participate in the same complex together with L4, they have distinct functions during DNA repair. L4 relies on X4 but not on Cernunnos for its stability, and L4 is required for optimal interaction of Cernunnos with X4. We demonstrate here, using in vitro-generated Cernunnos mutants and a series of functional assays in vivo, that the C-terminal region of Cernunnos is dispensable for its activity during DNA repair.

Domaines: Biochimie, Biologie Moléculaire

Laurent Malivert, Isabelle Callebaut, Paola Rivera-Munoz, Alain Fischer, Jean-Paul Mornon, et al.. The C-Terminal Domain of Cernunnos/XLF Is Dispensable for DNA Repair In Vivo. Molecular and Cellular Biology, 2009, 29 (5), pp.1116-1122. ⟨10.1128/MCB.01521-08⟩. ⟨hal-00361521⟩