Colloidal and physicochemical characterization of protein containing poly(lactide-co-glycolide) (PLGA) microspheres before and after drying

Abstract : In the present work, the double emulsion (W/O/W) method was used to microencapsulate a protein model (Bovine Serum Albumin) in a biodegradable polymer poly(lactide-co-glycolide) (50:50) (PLGA). Colloidal and physicochemical characterization of PLGA based microspheres was investigated after their “crude” preparation and after drying and redispersion processes. Particle morphology and size were studied by scanning electron microscopy and by laser diffraction analysis. Electrokinetic study and colloidal stability were also studied as a function of pH and salinity of the medium. Atomic force microscopy (AFM) was used to study the surface properties. It was found that smooth microspheres were obtained after the drying process. The Atomic force microscopy results of microspheres stored for one month in water show softness and surface irregularity caused by the microspheres degradation. The colloidal stability of theses microspheres is mainly governed by repulsive electrostatic interaction due to the PLGA charge. The used amount of stabilizing agent poly(vinyl alcohol) (PVA) is low enough to ensure the sterical stabilization. Drying process has no effect on the surface properties and colloidal stability of the prepared microspheres.
Type de document :
Article dans une revue
e-polymers, De Gruyter, 2009, 10, pp.1-12
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https://hal.archives-ouvertes.fr/hal-00358443
Contributeur : Nida Sheibat-Othman <>
Soumis le : mardi 3 février 2009 - 15:14:56
Dernière modification le : jeudi 19 avril 2018 - 14:34:08

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  • HAL Id : hal-00358443, version 1

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Nader Kalaji, Nida Sheibat-Othman, Hassan Saadaoui, Abdelhamid Elaissari, Hatem Fessi. Colloidal and physicochemical characterization of protein containing poly(lactide-co-glycolide) (PLGA) microspheres before and after drying. e-polymers, De Gruyter, 2009, 10, pp.1-12. 〈hal-00358443〉

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