Self-diffusion in chitosan networks : from a gel-gel method to fluorescence recovery after photobleaching by fringe pattern

Linda Payet (1) , Alain Ponton (1) , Liliane Léger (2, 3) , Hubert Hervet (2) , Jean-Louis Grossiord (4) , Florence Agnely (4)

1 MSC - Matière et Systèmes Complexes
2 LPFO - Laboratoire de physique des fluides organisés
3 LPS - Laboratoire de Physique des Solides
4 PCPB - Physico-chimie, pharmacotechnie, biopharmacie

The diffusion properties of caffeine and dextran within chitosan networks with controlled structure were investigated using a gel−gel method and fluorescence recovery after photobleaching (FRAP) by fringe pattern. The center of mass diffusion was studied by varying the molecular weight of dextran macromolecules labeled with fluorescein isothiocyanate (FITC). It was shown that the diffusion was drastically slowed down above a critical molecular weight around 105 g/mol.

Mots clés: Biopolymers; Carbohydrates; Diffusion; Molecules; Pharmaceuticals

Domaines: Biomécanique [physics.med-ph]; Biomécanique [physics.med-ph]

Linda Payet, Alain Ponton, Liliane Léger, Hubert Hervet, Jean-Louis Grossiord, et al.. Self-diffusion in chitosan networks : from a gel-gel method to fluorescence recovery after photobleaching by fringe pattern. Macromolecules, 2008, 41 (23), pp.9376-9381. ⟨10.1021/ma801192q⟩. ⟨hal-00353343⟩