Transforming growth factor-beta1 increases airway wound repair via MMP-2 upregulation: a new pathway for epithelial wound repair - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue American Journal of Physiology - Lung Cellular and Molecular Physiology Année : 2006

Transforming growth factor-beta1 increases airway wound repair via MMP-2 upregulation: a new pathway for epithelial wound repair

Résumé

In vivo, transforming growth factor (TGF)-beta1 and matrix metalloproteinases (MMPs) present at the site of airway injury are thought to contribute to epithelial wound repair. As TGF-beta1 can modulate MMP expression and MMPs play an important role in wound repair, we hypothesized that TGF-beta1 may enhance airway epithelial repair via MMPs secreted by epithelial cells. We evaluated the in vitro influence of TGF-beta1 on wound repair in human airway epithelial cells cultured under conditions allowing differentiation. The results showed that TGF-beta1 accelerated in vitro airway wound repair, whereas MMP inhibitors prevented this acceleration. In parallel, we examined the effect of TGF-beta1 on the expression of MMP-2 and MMP-9. TGF-beta1 induced a dramatic increase of MMP-2 expression with an increased steady-state level of MMP-2 mRNA, contrasting with a slight increase in MMP-9 expression. To confirm the role of MMP-2, we subsequently evaluated the effect of MMP-2 on in vitro airway wound repair and demonstrated that the addition of MMP-2 reproduced the acceleration of wound repair induced by TGF-beta1. These results strongly suggest that TGF-beta1 increases in vitro airway wound repair via MMP-2 upregulation. It also raises the issue of a different in vivo biological role of MMP-2 and MMP-9 depending on the cytokine microenvironment.
Fichier non déposé

Dates et versions

hal-00339598 , version 1 (18-11-2008)

Identifiants

Citer

Emmanuèle Lechapt-Zalcman, Virginie Prulière-Escabasse, David Advenier, Stéphane Galiacy, Cécile Charrière-Bertrand, et al.. Transforming growth factor-beta1 increases airway wound repair via MMP-2 upregulation: a new pathway for epithelial wound repair. American Journal of Physiology - Lung Cellular and Molecular Physiology, 2006, 290, pp.L1277-82. ⟨10.1152/ajplung.00149.2005⟩. ⟨hal-00339598⟩
119 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More