Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Nitrite-nitric oxide control of mitochondrial respiration at the frontier of anoxia.

Abstract : Actively respiring animal and plant tissues experience hypoxia because of mitochondrial O(2) consumption. Controlling oxygen balance is a critical issue that involves in mammals hypoxia-inducible factor (HIF) mediated transcriptional regulation, cytochrome oxidase (COX) subunit adjustment and nitric oxide (NO) as a mediator in vasodilatation and oxygen homeostasis. In plants, NO, mainly derived from nitrite, is also an important signalling molecule. We describe here a mechanism by which mitochondrial respiration is adjusted to prevent a tissue to reach anoxia. During pea seed germination, the internal atmosphere was strongly hypoxic due to very active mitochondrial respiration. There was no sign of fermentation, suggesting a down-regulation of O(2) consumption near anoxia. Mitochondria were found to finely regulate their surrounding O(2) level through a nitrite-dependent NO production, which was ascertained using electron paramagnetic resonance (EPR) spin trapping of NO within membranes. At low O(2), nitrite is reduced into NO, likely at complex III, and in turn reversibly inhibits COX, provoking a rise to a higher steady state level of oxygen. Since NO can be re-oxidized into nitrite chemically or by COX, a nitrite-NO pool is maintained, preventing mitochondrial anoxia. Such an evolutionarily conserved mechanism should have an important role for oxygen homeostasis in tissues undergoing hypoxia.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00338405
Contributor : Jacqueline Martin-Laffon Connect in order to contact the contributor
Submitted on : Thursday, November 13, 2008 - 10:05:37 AM
Last modification on : Friday, June 3, 2022 - 1:46:02 PM

Links full text

Identifiers

Citation

Abdelilah Benamar, Hardy Rolletschek, Ljudmilla Borisjuk, Marie-Hélène Avelange-Macherel, Gilles Curien, et al.. Nitrite-nitric oxide control of mitochondrial respiration at the frontier of anoxia.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 2008, 1777 (10), pp.1268-75. ⟨10.1016/j.bbabio.2008.06.002⟩. ⟨hal-00338405⟩

Share

Metrics

Record views

289