Skip to Main content Skip to Navigation
Journal articles

Genome-wide studies highlight indirect links between human replication origins and gene regulation.

Abstract : To get insights into the regulation of replication initiation, we systematically mapped replication origins along 1% of the human genome in HeLa cells. We identified 283 origins, 10 times more than previously known. Origin density is strongly correlated with genomic landscapes, with clusters of closely spaced origins in GC-rich regions and no origins in large GC-poor regions. Origin sequences are evolutionarily conserved, and half of them map within or near CpG islands. Most of the origins overlap transcriptional regulatory elements, providing further evidence of a connection with gene regulation. Moreover, we identify c-JUN and c-FOS as important regulators of origin selection. Half of the identified replication initiation sites do not have an open chromatin configuration, showing the absence of a direct link with gene regulation. Replication timing analyses coupled with our origin mapping suggest that a relatively strict origin-timing program regulates the replication of the human genome.
Document type :
Journal articles
Complete list of metadata

Cited literature [39 references]  Display  Hide  Download
Contributor : Martine Bondidier <>
Submitted on : Saturday, May 30, 2020 - 11:43:19 AM
Last modification on : Tuesday, July 20, 2021 - 5:20:05 PM


2008_Cadoret_Proceedings of th...
Publisher files allowed on an open archive



Jean-Charles Cadoret, Françoise Meisch, Vahideh Hassan-Zadeh, Isabelle Luyten, Claire Guillet, et al.. Genome-wide studies highlight indirect links between human replication origins and gene regulation.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2008, 105 (41), pp.15837-42. ⟨10.1073/pnas.0805208105⟩. ⟨hal-00332341⟩



Record views


Files downloads