Enantioselective acylation of R-2-pentanol in a solid/gas reactor catalysed by lipase B from Candida antarctica.

Abstract : The feasibility of the enzymatic enantioselective acylation of R-2-pentanol in a solid/gas reactor was demonstrated and compared to the same reaction carried out in liquid systems. This reaction was catalysed by lipase B from Candida antarctica (CALB) through transesterification of methyl propionate and R-2-pentanol. In the present study we show that there is no effect of the organic solvent on the enantioselectivity E of CALB for this reaction neither in the solid/gas system nor in organic liquid medium. The innovative idea of this work is the replacement of the substrates and solvent concentrations by their thermodynamic activities in order to correct the differences of substrates solvation in the different media studied and to control the level of availability of the different chemical species for the enzyme. Finally, we established that the solid/gas bioreactor is a suitable tool to study the influence of organic components on the enantioselectivity of lipases because it permits to control and adjust independently thermodynamic activities of substrates, on the one hand, and of an extra-added organic component on the other hand.
Document type :
Journal articles
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00329728
Contributor : Marianne Graber <>
Submitted on : Monday, October 13, 2008 - 11:54:28 AM
Last modification on : Tuesday, July 3, 2018 - 10:22:04 AM
Long-term archiving on: Monday, June 7, 2010 - 6:00:29 PM

File

Article_V._LEONARD_july_2004.p...
Files produced by the author(s)

Identifiers

  • HAL Id : hal-00329728, version 1

Collections

Citation

Valérie Léonard, Sylvain Lamare, Marie-Dominique Legoy, Marianne Graber. Enantioselective acylation of R-2-pentanol in a solid/gas reactor catalysed by lipase B from Candida antarctica.. Journal of Molecular Catalysis B: Enzymatic, Elsevier, 2004, 32, pp.53-59. ⟨hal-00329728⟩

Share

Metrics

Record views

847

Files downloads

531