Multicolor two-photon tissue imaging by wavelength mixing

Abstract : We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
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Submitted on : Wednesday, September 24, 2008 - 4:34:22 PM
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Pierre Mahou, Maxwell Zimmerley, Karine Loulier, Katherine Matho, Guillaume Labroille, et al.. Multicolor two-photon tissue imaging by wavelength mixing. Nature Methods, Nature Publishing Group, 2012, 9 (8), pp.815-818. ⟨10.1038/nmeth.2098⟩. ⟨hal-00324345⟩



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