Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues

Abstract : We use a vector field model to analyze the third-harmonic generation (THG) emission patterns for isolated objects illuminated by a Gaussian beam. Simulations and experiments indicate that THG from biological (dielectric) structures is essentially forward-directed, as opposed to e.g. THG from gold particles. We then address the issue of epidetecting forward-emitted light backscattered in a turbid medium. We use Monte Carlo simulations and measurements to analyze the effect of tissue properties (absorption, scattering), and of the geometry of the collecting optics. This analysis provides guidelines for optimizing epidetection in coherent nonlinear microscopy. An erratum is attached. © (2008) COPYRIGHT SPIE--The International Society for Optical Engineering.
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Submitted on : Wednesday, September 24, 2008 - 3:27:35 PM
Last modification on : Wednesday, January 23, 2019 - 10:28:59 AM

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Nicolas Olivier, Delphine Débarre, Emmanuel Beaurepaire. Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues. Biophotonics: Photonic Solutions for Better Health Care, Apr 2008, Strasbourg, France. ⟨10.1117/12.783376⟩. ⟨hal-00324271⟩

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