Rebinding of proximal histidine in the Cytochrome c' from Alcaligenes xylosoxidans acts as a molecular trap for nitric oxide

Byung-Kuk Yoo (1) , Jean-Louis Martin (1) , Colin R. Andrew (2) , Michel Négrerie (1)

1 LOB - Laboratoire d'optique et biosciences
2 Department of Chemistry and Biochemistry

Transient absorption spectra on cytochrome c' and their kinetics were recorded to identify the formation of 5-coordinate (5c)-NO and 5c-His hemes from 4c-heme (99% and 1% amplitudes; 7-ps and 100-ps time constants, respectively). We demonstrate that proximal histidine precludes NO rebinding at the proximal site.

Byung-Kuk Yoo, Jean-Louis Martin, Colin R. Andrew, Michel Négrerie. Rebinding of proximal histidine in the Cytochrome c' from Alcaligenes xylosoxidans acts as a molecular trap for nitric oxide. ULTRAFAST PHENOMENA XVI, Jun 2008, Stresa, Italy. pp.556-558, ⟨10.1007/978-3-540-95946-5_180⟩. ⟨hal-00324265⟩