Large-Scale, High-Resolution Data Acquisition System for Extracellular Recording of Electrophysiological Activity

K. Imfeld (1) , S. Neukom (2) , A. Maccione (3) , Y. Bornat (1, 4) , S. Martinoia (5) , P.-A. Farine (1) , M. Koudelka-Hep (1) , L. Berdondini (1, 5)

1 IMT - Institute of Microtechnology
2 CSEM - Centre Suisse d'Electronique et de Microtechnique SA [Neuchatel]
3 DIBE - Department of Biophysical and Electronic Engineering [Genoa]
4 IMS - Laboratoire de l'intégration, du matériau au système
5 IIT - Istituto Italiano di Tecnologia

A platform for high spatial and temporal resolution electrophysiological recordings of in vitro electrogenic cell cultures handling 4096 electrodes at a full frame rate of 8 kHz is presented and validated by means of cardiomyocyte cultures. Based on an active pixel sensor device implementing an array of metallic electrodes, the system provides acquisitions at spatial resolutions of 42 µmon an active area of 2.67mm×2.67 mm, and in the zooming mode, temporal resolutions down to 8 µs on 64 randomly selected electrodes. The low-noise performances of the integrated ampli- fier (11 µVrms) combined with a hardware implementation inspired by image/video processing concepts enable high-resolution acquisitions with real-time preprocessing capabilities adapted to the handling of the large amount of acquired data.

Mots clés: Active pixel sensor (APS) Cardiomyocytes culture Electrophysiology Extracellular recording High density High spatiotemporal resolution Microelectrode array Real-time processing

Domaines: Micro et nanotechnologies/Microélectronique; Ingénierie biomédicale

K. Imfeld, S. Neukom, A. Maccione, Y. Bornat, S. Martinoia, et al.. Large-Scale, High-Resolution Data Acquisition System for Extracellular Recording of Electrophysiological Activity. IEEE Transactions on Biomedical Engineering, 2008, 55 (8), pp.2064-2073. ⟨hal-00319351⟩