Recording and simulation of hippocampal neural networks with bicuculline

Guillaume Jean-Paul Claude Becq (1) , Sophie Roth (2, 3) , Steeve Zozor (4) , Jacques S. Brocard (3) , Sylvie Gory-Fauré (3) , Pierre-Olivier Amblard (4) , Catherine Villard (2)

1 GIPSA-Services - GIPSA-Services
2 NEEL - TPS - Thermodynamique et biophysique des petits systèmes
3 GIN - Grenoble Institut des Neurosciences
4 GIPSA-SIGMAPHY - GIPSA - Signal Images Physique

In this study we compare the neural activity of a population of neurons recorded with a MEA to simulations of equivalent networks obtained on a computer when bicuculline, an antagonist of inhibitory connections, is introduced into the nutritive solution. The aim of this study is to obtain a model producing extra-cellular data that match the synchronicity of two different real networks: a cell culture and an oraganotypic hippocampus slice. A one compartment model of neuron and a neuron-electrode model are used to simulate experiments. Parameters of the models are fitted to match in vivo data. It is shown that the variation of the noise level at the synaptic level induces a variation in the period of the bursting effect of bicuculline and produces variation in amplitude of the recorded signal.

Mots clés: stochastic resonance; neuron-electrode coupling; hippocampus; Izhikevich model; Microelectrode arrays

Domaines: Réseau de neurones [cs.NE]; Micro et nanotechnologies/Microélectronique

Guillaume Jean-Paul Claude Becq, Sophie Roth, Steeve Zozor, Jacques S. Brocard, Sylvie Gory-Fauré, et al.. Recording and simulation of hippocampal neural networks with bicuculline. MEA 2008 - 6th international meeting on substrate-integrated micro electrode arrays, Jul 2008, Reutlingen, Germany. pp.345-348. ⟨hal-00317573⟩