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Article Dans Une Revue Journal of Microbiological Methods Année : 2008

A novel and rapid method for synthesizing positive controls and standards for quantitative PCR

Maude M. David
  • Fonction : Auteur
Pascal Simonet
Timothy M Vogel
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Résumé

We developed and tested a method to produce DNA standards and controls for quantitative PCR by designing and performing partial hybridization of long oligonucleotides before double stranded DNA fragments were synthesized and subsequently amplified by conventional PCR. This approach does not require any natural DNA template. Applications include the production of standards, which cannot be easily produced from DNA extracted from bacteria or plants.

Dates et versions

hal-00313788 , version 1 (27-08-2008)

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Maude M. David, Amy R. Sapkota, Pascal Simonet, Timothy M Vogel. A novel and rapid method for synthesizing positive controls and standards for quantitative PCR. Journal of Microbiological Methods, 2008, 73 (1), pp.73-77. ⟨10.1016/j.mimet.2008.01.001⟩. ⟨hal-00313788⟩
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