Regulation of growth, protein synthesis, and maturation of fetal bovine epiphyseal chondrocytes grown in high-density culture in the presence of ascorbic acid, retinoic acid, and dihydrocytochalasin B.

Anne-Marie Freyria (1) , Marie-Claire Ronzière (1) , S. Roche (1) , Cécile F. Rousseau (1) , Daniel Herbage (1)

1 IBCP - Institut de biologie et chimie des protéines [Lyon]

Phenotypic expression of chondrocytes can be modulated in vitro by changing the culture technique and by agents such vitamins and growth factors. We studied the effects of ascorbic acid, retinoic acid (0.5 and 10 μM), and dihydrocytochalasin B (3, 10, 20 μM DHCB), separately or in combination (ascorbic acid + retinoic acid or ascorbic acid + DHCB), on the induction of maturation of fetal bovine epiphyseal chondrocytes grown for up to 4 weeks at high density in medium containing 10% fetal calf serum and the various agents. In the absence of any agent or with retinoic acid or DHCB alone, the metabolic activity of the cells remained very low after day 6, with no induction of type I or X collagen synthesis nor increase in alkaline phosphatase activity. Chondrocytes treated with fresh ascorbic acid showed active protein synthesis associated with expression of types I and X after 6 and 13 days, respectively. This maturation was not accompanied by obvious hypertrophy of the cells or high alkaline phosphatase activity. Addition of retinoic acid to the ascorbic acid-treated cultures decreased the level of type II collagen synthesis and delayed the induction of types I and X collagen, which were present only after 30 days. A striking increase in alkaline phosphatase activity (15–20-fold) was observed in the presence of both ascorbic acid and the highest dose of retinoic acid (10 μM). DHCB was also a potent inhibitor of the maturation induced by treatment with ascorbic acid, as the chondrocytes maintained their rounded shape and synthesized type II collagen without induction of type I or X collagen. The pattern of protein secretion was compared under all culture conditions by two-dimensional gel electrophoresis. The different regulations of chondrocyte differentiation by ascorbic acid, retinoic acid, and DHCB were confirmed by the important qualitative and quantitative changes in the pattern of secreted proteins observed by two-dimensional gel electrophoresis along the study.

Mots clés: Proteomics; Bovine chondrocytes; Type II collagen; Cartilage; Chondrocyte maturation; Type I collagen; Type X collagen; Ascorbate; Retinoic acid; Dihydrocytochalasin B; 2D-PAGE

Domaines: Biochimie, Biologie Moléculaire

Anne-Marie Freyria, Marie-Claire Ronzière, S. Roche, Cécile F. Rousseau, Daniel Herbage. Regulation of growth, protein synthesis, and maturation of fetal bovine epiphyseal chondrocytes grown in high-density culture in the presence of ascorbic acid, retinoic acid, and dihydrocytochalasin B.. Journal of Cellular Biochemistry, 1999, 76 (1), pp.84-98. ⟨10.1002/(SICI)1097-4644(20000101)76:1<84::AID-JCB9>3.0.CO;2-Z⟩. ⟨hal-00313321⟩