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Strategy for in situ detection of natural transformation-based horizontal gene transfer events

Abstract : A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.
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https://hal.archives-ouvertes.fr/hal-00312918
Contributor : Publications Ampère <>
Submitted on : Tuesday, August 26, 2008 - 11:28:38 PM
Last modification on : Friday, December 11, 2020 - 12:34:02 PM

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Aurora Rizzi, Alessandra Pontiroli, Lorenzo Brusetti, Sara Borin, Claudia Sorlini, et al.. Strategy for in situ detection of natural transformation-based horizontal gene transfer events. Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (4), pp.1250-1254. ⟨10.1128/AEM.02185-07⟩. ⟨hal-00312918⟩

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