Asymmetric tethering of flat and curved lipid membranes by a golgin.

Guillaume Drin (1) , Vincent Morello (1) , Jean-François Casella (1) , Pierre Gounon (2) , Bruno Antonny (1)

1 IPMC - Institut de pharmacologie moléculaire et cellulaire
2 CCMA - Centre Commun de Microscopie Appliquée

Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP-210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP-210 and in ArfGAP1, which controlled the interaction of the C terminus of GMAP-210 with the small guanine nucleotide-binding protein Arf1. Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow.

Domaines: Biochimie, Biologie Moléculaire

Guillaume Drin, Vincent Morello, Jean-François Casella, Pierre Gounon, Bruno Antonny. Asymmetric tethering of flat and curved lipid membranes by a golgin.. Science, 2008, 320 (5876), pp.670-3. ⟨10.1126/science.1155821⟩. ⟨hal-00311958⟩