Skip to Main content Skip to Navigation
Journal articles

Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray.

Abstract : Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray analysis to identify mRNAs that are regulated by changes in their adenylation state during oogenesis and early development of the diploid frog Xenopus tropicalis. The microarray data were validated using qRT-PCR and direct analysis of the adenylation state of endogenous maternal mRNAs during the period studied. We identified more than 500 mRNAs regulated at the post-transcriptional level among the 3000 mRNAs potentially detected by the microarray. The mRNAs were classified into nine different adenylation behavior categories. The various adenylation profiles observed during oocyte maturation and early development and the analyses of 3'-untranslated region sequences suggest that previously uncharacterized sequence elements control the adenylation behavior of the newly identified mRNAs. These data should prove useful in identifying mRNAs with important functions during oocyte maturation and early development.
Document type :
Journal articles
Complete list of metadata

Cited literature [63 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00289723
Contributor : Laurence Petit Le Cadre Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 10:11:58 PM
Last modification on : Thursday, January 14, 2021 - 11:21:29 AM

File

2006_Graindorge_Nucleic Acids ...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Citation

Antoine Graindorge, Raphaël Thuret, Nicolas Pollet, Howard Beverley Osborne, Yann Audic. Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray.. Nucleic Acids Research, Oxford University Press, 2006, 34 (3), pp.986-95. ⟨10.1093/nar/gkj492⟩. ⟨hal-00289723⟩

Share

Metrics

Record views

292

Files downloads

93