The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.

Abstract : Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00277601
Contributor : Malcolm Buckle <>
Submitted on : Tuesday, May 6, 2008 - 4:29:29 PM
Last modification on : Monday, January 13, 2020 - 5:08:14 PM

Links full text

Identifiers

Collections

Citation

F. Brunel, M. M. Zakin, H. Buc, M. Buckle. The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.. Nucleic Acids Research, Oxford University Press, 1996, 24 (9), pp.1608-15. ⟨10.1093/nar/24.9.1608⟩. ⟨hal-00277601⟩

Share

Metrics

Record views

128