Matrix merging arrangements for the study of protein dynamics by time-resolved step-scan Fourier transform infrared spectroscopy and multivariate curve resolution

C. Ruckebusch (1) , L. Duponchel (1) , J.P. Huvenne (1) , J. Saurina (2)

1 LASIRE - Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516
2 Department of Analytical Chemistry

In this paper, we propose the construction of merging arrangements for combining the information from various runs as a powerful approach to improve the resolution. The bacteriorhodopsin (bR) photocycle has been chosen in this study as an example dealing with the protein dynamics monitored by means of time-resolved step-scan FT-IR spectroscopy. The possibilities of matrix merging are evaluated and results are compared with those from the analysis of individual and augmented matrices. As a conclusion, this strategy provides excellent results for the analysis of this type of time-resolved FT-IR data.

C. Ruckebusch, L. Duponchel, J.P. Huvenne, J. Saurina. Matrix merging arrangements for the study of protein dynamics by time-resolved step-scan Fourier transform infrared spectroscopy and multivariate curve resolution. Analytica Chimica Acta, 2004, 515, pp.183-190. ⟨10.1016/j.aca.2003.11.062⟩. ⟨hal-00269269⟩