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Phosphatidylcholine-specific phospholipase C but not gamma interferon regulate gene expression and secretion of CC Chemokine Ligand-2 (CCL-2) by human astrocytes during infection by Toxoplasma gondii.

Abstract : We have used human astrocytoma-derived cells to investigate the cellular responses of central nervous system cells to Toxoplasma gondii infection. At 24 h post inoculation, the secretion of CCL-2 (or Monocyte Chemotactic Protein-1) was augmented six-fold over the control. This secretion was down-regulated by D609, a specific inhibitor of phosphatidylcholine-dependent phospholipase C (PC-PLC), but not modulated by gamma interferon (IFN-gamma). Ribonuclease protection assay analyses showed significant down-regulation of CCL-2 mRNA production during infection by Toxoplasma gondii when cells were treated by D609. The mRNA levels of the seven other chemokines studied were not modified by D609. CCL-2 seems to contribute to the cell recruitment during human cerebral reactivation of Toxoplasma gondii. Cellular production of this CC chemokine during toxoplasmosis may be regulated by a PC-PLC-dependent pathway.
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https://hal.archives-ouvertes.fr/hal-00267385
Contributor : Jean Gagnon Connect in order to contact the contributor
Submitted on : Thursday, March 27, 2008 - 1:13:48 PM
Last modification on : Friday, June 25, 2021 - 7:22:01 PM

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François Durand, Marie-Pierre Brenier-Pinchart, Francois Berger, Patrice N Marche, Renée Grillot, et al.. Phosphatidylcholine-specific phospholipase C but not gamma interferon regulate gene expression and secretion of CC Chemokine Ligand-2 (CCL-2) by human astrocytes during infection by Toxoplasma gondii.. Parasite Immunology, Wiley, 2004, 26 (10), pp.419-22. ⟨10.1111/j.0141-9838.2004.00722.x⟩. ⟨hal-00267385⟩

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