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NIMA-related kinase TbNRKC is involved in basal body separation in Trypanosoma brucei.

Abstract : The NIMA-related kinase 2 (NEK 2) has important cell cycle functions related to centriole integrity and splitting. Trypanosoma brucei does not possess centrioles, however, cytokinesis is coupled to basal body separation events. Here we report the first functional characterisation of a T. brucei basal body-cytoskeletal NIMA-related kinase (NRK) protein, TbNRKC. The TbNRKC kinase domain has high amino acid identity with the human NEK1 kinase domain (50%) but also shares 42% identity with human NEK2. TbNRKC is expressed in bloodstream and procyclic cells and functions as a bona fide kinase in vitro. Remarkably, RNAi knockdown of TbNRKC and overexpression of kinase-dead TbNRKC in procyclic forms induces the accumulation of cells with four basal bodies, whereas overexpression of active protein produces supernumary basal bodies and blocks cytokinesis. TbNRKC is located on mature and immature basal bodies and is the first T. brucei NRK to be found associated with the basal body cytokinesis pathway.
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Submitted on : Thursday, January 24, 2008 - 9:53:36 AM
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Lydie C Pradel, Mélanie Bonhivers, Nicolas Landrein, Derrick R Robinson. NIMA-related kinase TbNRKC is involved in basal body separation in Trypanosoma brucei.. Journal of Cell Science, Company of Biologists, 2006, 119 (Pt 9), pp.1852-63. ⟨10.1242/jcs.02900⟩. ⟨hal-00215921⟩



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