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Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.

Abstract : Two forms of RNA Polymerase IV (PolIVa/PolIVb) have been implicated in RNA-directed DNA methylation (RdDM) in Arabidopsis. Prevailing models imply a distinct function for PolIVb by association of Argonaute4 (AGO4) with the C-terminal domain (CTD) of its largest subunit NRPD1b. Here we show that the extended CTD of NRPD1b-type proteins exhibits conserved Argonaute-binding capacity through a WG/GW-rich region that functionally distinguishes Pol IVb from Pol IVa, and that is essential for RdDM. Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally conserved Argonaute-binding platforms in RNA interference (RNAi)-related components.
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https://hal.archives-ouvertes.fr/hal-00198842
Contributor : Jean Gagnon Connect in order to contact the contributor
Submitted on : Tuesday, December 18, 2007 - 9:13:57 AM
Last modification on : Wednesday, March 16, 2022 - 4:06:01 PM

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Mahmoud El-Shami, Dominique Pontier, Sylvie Lahmy, Laurence Braun, Claire Picart, et al.. Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.. Genes and Development, Cold Spring Harbor Laboratory Press, 2007, 21 (20), pp.2539-44. ⟨10.1101/gad.451207⟩. ⟨hal-00198842⟩

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