PDMS-based microfluidics for proteomic analysis.

Arash Dodge (1) , Edouard Brunet (1, 2, 3, 4, 5) , Suelin Chen (1, 6, 7, 8, 9, 10, 11) , Jacques Goulpeau (1) , Valérie Labas (1, 12) , Joelle Vinh (1, 12) , Patrick Tabeling (1, 2, 13, 3)

1 ESPCI Paris - Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris
2 LPS - Laboratoire de Physique Statistique de l'ENS
3 MMN - Laboratoire Microfluidique, MEMS, Nanostructures
4 RUNTIME - Efficient runtime systems for parallel architectures
5 AUF - Agence Universitaire de la Francophonie
6 DEPARTMENT OF MICROBIOLOGY AND NATIONAL KEY LABORATORY FOR AGROBIOTECHNOLOGY - Department of Microbiology and National Key Laboratory for Agrobiotechnology
7 SPB - School of Plant Biology
8 AI-ECON Research Center
9 CEDAR - Center of Excellence for Document Analysis and Recognition
10 Gilead Sciences
11 HKUST - Hong Kong University of Science and Technology
12 NDC - Neurobiologie et diversité cellulaire
13 LPCT - Laboratoire de Physico-Chimie Théorique

A microfluidic poly(dimethylsiloxane) (PDMS) microdevice was realized, combining on-line protein electrophoretic separation, selection, and digestion of a protein of interest for identification by mass spectrometry. The system includes eight integrated valves and one micropump dedicated to control the flow operations. Myoglobin was successfully isolated from bovine serum albumin (BSA), then selected using integrated valves and digested in a rotary micromixer. Proteolytic peptides were recovered from the micromixer for protein identification. Total analysis from sample injection to protein identification is performed under 30 minutes, with samples of tens of nanolitres. The paper shows that PDMS technology can be successfully used for integrating complex preparation protocols of proteic samples prior to MS analysis.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Arash Dodge, Edouard Brunet, Suelin Chen, Jacques Goulpeau, Valérie Labas, et al.. PDMS-based microfluidics for proteomic analysis.. Analyst, 2006, 131 (10), pp.1122-8. ⟨10.1039/b606394b⟩. ⟨hal-00196106⟩