Gangliosides from human melanoma tumors impair dendritic cell differentiation from monocytes and induce their apoptosis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Immunology Année : 2003

Gangliosides from human melanoma tumors impair dendritic cell differentiation from monocytes and induce their apoptosis.

Josette Péguet-Navarro
  • Fonction : Auteur
Myriam Sportouch
  • Fonction : Auteur
Iuliana Popa
  • Fonction : Auteur
Daniel Schmitt
  • Fonction : Auteur
Jacques Portoukalian
  • Fonction : Auteur

Résumé

Gangliosides are ubiquitous membrane-associated glycosphingolipids, which are involved in cell growth and differentiation. Most tumor cells synthesize and shed large amounts of gangliosides into their microenvironment, and many studies have unraveled their immunosuppressive properties. In the present study we analyzed the effects of GM3 and GD3 gangliosides, purified from human melanoma tumors, on the differentiation of monocyte-derived dendritic cells (MoDC). At concentrations close to those detected in the sera from melanoma patients, both gangliosides dose-dependently inhibit the phenotypic and functional differentiation of MoDC, as assessed by a strong down-regulation of CD1a, CD54, CD80, and CD40 Ags and impaired allostimulatory function on day 6 of culture. Furthermore, GM3 and GD3 gangliosides decreased the viable cell yield and induced significant DC apoptosis. Finally, addition of GD3 to differentiating DC impaired their subsequent maturation induced by CD154. The resulting DC produced low amounts of IL-12 and large amounts of IL-10, a cytokine pattern that might hamper an efficient antitumor immune response. In conclusion, the results demonstrate that gangliosides impair the phenotypic and functional differentiation of MoDC and induce their apoptosis, which may be an additional mechanism of human melanoma escape.
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Dates et versions

hal-00189862 , version 1 (22-11-2007)

Identifiants

  • HAL Id : hal-00189862 , version 1
  • PUBMED : 12646609

Citer

Josette Péguet-Navarro, Myriam Sportouch, Iuliana Popa, Odile Berthier, Daniel Schmitt, et al.. Gangliosides from human melanoma tumors impair dendritic cell differentiation from monocytes and induce their apoptosis.. Journal of Immunology, 2003, 170 (7), pp.3488-94. ⟨hal-00189862⟩
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