Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.

Vitaly E Syakhovich , N. T. Saraswathi , Marc Ruff (1) , Sergey B Bokut , Dino Moras (1)

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 A at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 A, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 9.70 A3 Da(-1) and a solvent content of 49%.

Domaines: Biologie moléculaire

Vitaly E Syakhovich, N. T. Saraswathi, Marc Ruff, Sergey B Bokut, Dino Moras. Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.. Acta crystallographica Section F : Structural biology communications [2014-..], 2006, 62 (Pt 2), pp.106-9. ⟨10.1107/S1744309105042764⟩. ⟨hal-00188157⟩