Peptide immobilization on amine-terminated boron-doped diamond surfaces.

Leon Gengembre (1) , Rabah Boukherroub (2, 3) , Yannick Coffinier (2, 3) , Didier Delabouglise (4) , Remi Desmet (5) , Charafeddine Jama (6) , Bernadette Marcus (4) , Oleg Melnyk (5) , Sabine Szunerits (4) , E. Payen (1)

1 UCCS - Unité de Catalyse et Chimie du Solide - UMR 8181
2 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
3 IRI - Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq]
4 LEPMI - Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces
5 Institut de Biologie
6 PERF - Laboratoire des Procédés d'Elaboration des Rvêtements Fonctionnels

This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further prepn. of peptide microarray through site-specific a-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 plasma treatment and then reacted with triphosgene and Fmoc-protected (Fmoc = 9-fluorenylmethoxycarbonyl) hydrazine to yield a protected semicarbazide termination. Subsequent deprotection and chem. reaction with glyoxylyl peptides led to the covalent immobilization of the peptides on the surface through site-specific ligation. The resulting surfaces were characterized using XPS and fluorescence measurements.

Domaines: Matériaux; Chimie organique

Leon Gengembre, Rabah Boukherroub, Yannick Coffinier, Didier Delabouglise, Remi Desmet, et al.. Peptide immobilization on amine-terminated boron-doped diamond surfaces.. Langmuir, 2007, 23 (8), pp.4494-4497. ⟨10.1021/la063440y⟩. ⟨hal-00179759⟩