Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.

Christian B F Andersen (1) , Lionel Ballut (2, 1) , Jesper S Johansen (1) , Hala Chamieh (2) , Klaus H Nielsen (1) , Cristiano L P Oliveira (1) , Jan Skov Pedersen (1) , Bertrand Séraphin (2) , Hervé Le Hir (2) , Gregers Rom Andersen (1)

1 Aarhus University [Aarhus]
2 CGM - Centre de génétique moléculaire

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

Domaines: Biochimie, Biologie Moléculaire; Biologie moléculaire

Christian B F Andersen, Lionel Ballut, Jesper S Johansen, Hala Chamieh, Klaus H Nielsen, et al.. Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.. Science, 2006, 313 (5795), pp.1968-72. ⟨10.1126/science.1131981⟩. ⟨hal-00168014⟩