Skip to Main content Skip to Navigation
Journal articles

Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.

Abstract : In Caenorhabditis elegans, the Mex-3 protein is a translational regulator that specifies the posterior blastomere identity in the early embryo and contributes to the maintenance of the germline totipotency. We have now identified a family of four homologous human Mex-3 genes, called hMex-3A to -3D that encode proteins containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. The hMex-3 are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1-dependent export pathway. Our analysis further revealed that hMex-3A and hMex-3B, but not hMex-3C, colocalize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover. Taken together, these findings indicate that hMex-3 proteins constitute a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00167447
Contributor : Nathalie Gibelin <>
Submitted on : Monday, August 20, 2007 - 5:00:50 PM
Last modification on : Tuesday, July 20, 2021 - 5:20:05 PM

Links full text

Identifiers

Citation

Karine Buchet-Poyau, Julien Courchet, Hervé Le Hir, Bertrand Séraphin, Jean-Yves Scoazec, et al.. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.. Nucleic Acids Research, Oxford University Press, 2007, 35 (4), pp.1289-300. ⟨10.1093/nar/gkm016⟩. ⟨hal-00167447⟩

Share

Metrics

Record views

415