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Communication Dans Un Congrès Année : 2002

Analyse directe de neuropeptides dans le cerveau de rat par spectrométrie de masse MALDI-TOF In Situ

Résumé

The measure of neuropeptides is a important tool in biology to better define endocrine and neuroendocrine function. Traditionally most methods have relied on the development of specific antibodies. Newer molecular methodologies have used measures of gene expression of neuropeptide precursors, such as Northern blot, PCR or in situ hybridization analysis. Matrix-assisted laser desorption/ionization (MALDI) mass analysis is a novel powerful technique for investigation of neuropeptides. Multiple peptides and peptide forms can be detected simultaneously and with great sensitivity in tissue extracts or partially purified samples. We have now adapted a MALDI methodology for the direct measurement of neuropeptides on fresh rat brain tissue sections. We have validated the method by examining peptidergic mass profiles of the supraoptic nucleus (SON) and caudate putamen hypothalamic regions. Interestingly, mass profiles shown that vasopressin which is specifically present in the SON is modulated when animals are treated with lipopolysaccharides. MALDI-MS on brain slides is a novel complementary technique for neurobiologists and endocrinologists in order to investigate the dynamic and regional repartition of neuropeptides during physiological events.
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Dates et versions

hal-00167328 , version 1 (21-08-2007)

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  • HAL Id : hal-00167328 , version 1

Citer

R. Day, I. Fournier, M. Salzet. Analyse directe de neuropeptides dans le cerveau de rat par spectrométrie de masse MALDI-TOF In Situ. Societé Française de Spectrométrie de masse, club jeune, 2002, Chaville, France. pp.0. ⟨hal-00167328⟩

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