USE OF CONJUGATES WITH LINKERS CLEAVABLE BY PHOTODISSOCIATION OR FRAGMENTATION FOR MASS SPECTROMETRY ANALYSIS OF TISSUE SECTIONS

R. Lemaire (1) , I. Fournier (1) , M. Salzet (1) , M. Deschamps (2) , Jc. Tabet (3) , G. Proess (2) , I. Rudloff (2) , M. Lemaitre (2)

1 NA - Neuroimmunologie des annélides
2 UROGENTEC
3 SSFMB - Synthèse, Structure et Fonction de Molécules Bioactives

The invention concerns a method for determining at least one target molecule map in a tissue section, using at least one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least l,and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV- MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization.

Mots clés: MALDI imaging; specific imaging; Tag mass; mass sepctrometry; diagnosis

Domaines: Chimie analytique; Biochimie, Biologie Moléculaire; Bio-Informatique, Biologie Systémique [q-bio.QM]; Bio-informatique [q-bio.QM]; Médecine humaine et pathologie; Sciences pharmaceutiques; Cancer; Ingénierie biomédicale

R. Lemaire, I. Fournier, M. Salzet, M. Deschamps, Jc. Tabet, et al.. USE OF CONJUGATES WITH LINKERS CLEAVABLE BY PHOTODISSOCIATION OR FRAGMENTATION FOR MASS SPECTROMETRY ANALYSIS OF TISSUE SECTIONS. United States, Patent n° : WO2006IB02309 20060607. 2007, pp.65. ⟨hal-00167310⟩