Tag-mass: Specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag

R. Lemaire (1) , J. Stauber (1) , M. Wisztorski (1) , C. van Camp (1) , A. Desmons (1) , M. Deschamps (2) , G. Proess (2) , I. Rudlof (2) , A. S. Woods (3) , R. Day (4) , M. Salzet (1) , I. Fournier (1)

1 NA - Neuroimmunologie des annélides
2 UROGENTEC
3 NIDA - National Institute on Drug Abuse [NIH, Bethesda, USA]
4 Département de Pharmacologie

MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.

Mots clés: MALDI Imaging; Tag-mass; Mass spectrometry

Domaines: Chimie analytique; Biochimie, Biologie Moléculaire; Ingénierie biomédicale

R. Lemaire, J. Stauber, M. Wisztorski, C. van Camp, A. Desmons, et al.. Tag-mass: Specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag. Journal of Proteome Research, 2007, 6 (6), pp.2057-2067. ⟨10.1021/pr0700044⟩. ⟨hal-00167235⟩