Circulating antibodies to nitrosylated proteins: a biomarker for familial amyotrophic lateral sclerosis? Study on the hSOD1 transgenic rat model. - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2006

Circulating antibodies to nitrosylated proteins: a biomarker for familial amyotrophic lateral sclerosis? Study on the hSOD1 transgenic rat model.

C. Nicaise
  • Fonction : Auteur
A. Boom
  • Fonction : Auteur
C. van Der Velden
  • Fonction : Auteur
M.P. Dabadie
  • Fonction : Auteur
R. Pochet
  • Fonction : Auteur

Résumé

Background Multifactorial processes have been proposed to contribute to amyotrophic lateral sclerosis (ALS), as oxidative stress through acquisition of toxic properties of the mutant superoxide dismutase 1 (SOD1). Recently, a role for oxidative damage on proteins has been proposed based on the enhanced production of free and bound nitrotyrosine in the cerebrospinal fluid of ALS human patients and murine models. Other biomarkers, as oxidative products in the blood including nitrosylated proteins or epitopes, could be useful in the diagnosis of ALS. Aim We investigated the presence of circulating antibodies directed against nitrosylated amino acids on plasmatic proteins as biological indicators of nitric oxide (NO) production and ALS disease progression. Methods We used hSOD1 (G93A) transgenic rats from Taconic and the age- and gender- matched wild-type littermates as controls. Rats were weighted every day and a clinical score was measured. Plasma was obtained on 10% citrated tube by tail venipunction. Enzyme-linked immunosorbent assays were performed on the blood samples to assay circulating antibodies directed to NO-Asparagine, NO-Methionine, NO-Histidine, NO-Tyrosin, NO-Arginine, NO-Phenylalanine and NO-Tryptophan. Results In ALS rat plasma, we have found higher levels of some antibodies directed against nitrosylated amino acids compared to control rat: NO-Asparagine (0, 158±0, 008 versus 0, 120±0, 011 units of Optical Density, p<0, 05), NO-Methionine (0, 224±0, 019 versus 0, 131±0, 040 units of O. D., p<0, 05) and NO-Histidine (0, 204±0, 021 versus 0, 081±0, 027 units of O. D., p<0, 05) circulating antibodies are significantly increased in ALS rat during the end of life. No striking difference was observed for NO-Tyrosin, NO-Arginine, NO-Phenylalanine or NO-Tryptophan. Conclusion Circulating anti-NO-Asparagine, NO-Methionine and NO-Histidine antibodies could be used as blood biomarkers in a rat model of amyotrophic lateral sclerosis to assess the disease progression
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Dates et versions

hal-00162053 , version 1 (12-07-2007)

Identifiants

  • HAL Id : hal-00162053 , version 1

Citer

C. Nicaise, A. Boom, C. van Der Velden, M.P. Dabadie, M. Geffard, et al.. Circulating antibodies to nitrosylated proteins: a biomarker for familial amyotrophic lateral sclerosis? Study on the hSOD1 transgenic rat model.. FENS Forum, Jul 2006, Vienne, Austria. ⟨hal-00162053⟩
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