Identification of a Vibrio strain producing antimicrobial activities in the excretory organs of Nautilus pompilius (Cephalopoda, Nautiloidea). - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Reviews in Fish Biology and Fisheries Année : 2007

Identification of a Vibrio strain producing antimicrobial activities in the excretory organs of Nautilus pompilius (Cephalopoda, Nautiloidea).

Résumé

The aim of the present study was to identify and characterize bacteria producing antimicrobial compounds in the excretory organs of Nautilus pompilius. Culture-dependent and culture-independent complementary approaches were used for bacterial identification such as: culture on selective media, Gram staining, CARD-FISH, direct DNA extraction from host tissue, PCR amplification and sequencing of the bacterial 16S rRNA gene. Results show presence of three bacterial groups: gamma-Proteobacteria with three clusters (Pseudomonadales, Vibrionales, Alteromonadales), beta-Proteobacteria and spirochetes. In order to screen for active strains, antimicrobial activity was tested by diffusion agar assay against Micrococcus luteus, Escherichia coli, Vibrio harveyi, and Candida albicans. Nautilus isolates showed antimicrobial activities against both Gram-positive and Gram-negative reference strains. Most of the active strains were phylogenetically related to environmental Vibrionaceae. These strains were always abundant in N. pompilius PA but were absent from Nautilus macromphalus from other geographical areas. Therefore, we suggest that antimicrobial active Vibrionaceae infect N. pompilius by environmental transmission.

Dates et versions

hal-00146199 , version 1 (14-05-2007)

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Mathieu Pernice, D. Destoumieux-Garzon, Jean Peduzzi, Sylvie Rebuffat, Renata Boucher-Rodoni. Identification of a Vibrio strain producing antimicrobial activities in the excretory organs of Nautilus pompilius (Cephalopoda, Nautiloidea).. Reviews in Fish Biology and Fisheries, 2007, 17, pp.197-205. ⟨10.1007/s11160-006-9029-3⟩. ⟨hal-00146199⟩
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