Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Abstract : Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here,we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
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https://hal.archives-ouvertes.fr/hal-00144490
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Submitted on : Thursday, May 3, 2007 - 3:09:52 PM
Last modification on : Thursday, February 7, 2019 - 2:31:05 PM

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Edward J. Botcherby, Christopher W. Smith, Michael Kohl, Delphine Débarre, Martin J. Booth, et al.. Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2011, 109 (8), pp.2919-2924. ⟨10.1073/pnas.1111662109⟩. ⟨hal-00144490⟩

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