Quantification of sudden light-induced polarization in bacteriorhodopsin by optical rectification

Abstract : Upon population of its excited state, the retinal chromophore in the membrane protein bacteriorhodopsin (bR) undergoes a sudden (less than 10 fs) change in dipole moment, Δμ, that can be visualized in a direct way by optical rectification of a broadband visible femtosecond light pulse to the infrared but has not been quantified in this way. Here we show that a transparent thick AgGaS2 crystal delivers infrared radiation with the same spectral profile as bR and is a suitable reference for quantifying conversion efficiency. Using this reference, we estimate the projection of Δμ on the membrane normal at 11 D, corresponding to the displacement of a full charge over approximately half the length of the retinal chromophore. This result may help to evaluate models describing the interplay between the initial polarization change and the subsequent isomerization of the retinal.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00144489
Contributor : Laure Lachapelle <>
Submitted on : Thursday, May 3, 2007 - 3:07:41 PM
Last modification on : Thursday, February 7, 2019 - 4:03:58 PM

Identifiers

Collections

Citation

Anne Colonna, Geza Groma, Jean-Louis Martin, Manuel Joffre, Marten Vos. Quantification of sudden light-induced polarization in bacteriorhodopsin by optical rectification. Journal of Physical Chemistry B, American Chemical Society, 2007, 111 (10), pp.2707-2710. ⟨10.1021/jp0673462⟩. ⟨hal-00144489⟩

Share

Metrics

Record views

127