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Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane

Abstract : A novel formaldehyde sensitive biosensor based on bacterial formaldehyde dehydrogenase (FDH) as a bio-recognition element has been developed. The bio-recognition membrane had bi-layer architecture and consisted of FDH, cross-linked with albumin, and of the cofactor NAD at a high concentration level (first layer). The second layer was a negatively charged Nafion membrane, which prevented a leakage of negatively charged NAD molecules from the bio-membrane. As transducers, gold electrodes SiO2/Si/SiO2/Ti/Au and electrolyte-insulator-semiconductor Si/SiO2 (EIS) structures have been used. Changes in capacitance and impedance properties of the bio-recognition membrane have been used for monitoring formaldehyde concentration in a bulk solution. It has been shown that formaldehyde can be detected within a concentration range from 1 mu M to 20 mM depending on the type of transduction used, with a detection limit of 1 and 100 mu M for gold-based and EIS-based transducers, respectively.
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https://hal.archives-ouvertes.fr/hal-00140600
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Submitted on : Saturday, April 7, 2007 - 11:56:32 AM
Last modification on : Monday, September 13, 2021 - 2:44:02 PM

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  • HAL Id : hal-00140600, version 1
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Mounir Ben Ali, Y. Korpan, M. Gonchar, Anna El'Skaya, Mhamed Maaref, et al.. Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane. Biosensors and Bioelectronics, Elsevier, 2006, 22 (5), pp.575-581. ⟨hal-00140600⟩

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